Shri Radha Mohan Singh releases recombinant ELISA kits

9th January, 2018, Krishi Bhavan, New Delhi

Shri Radha Mohan Singh, Union Minister of Agriculture & Farmers Welfare, released the recombinant ELISA kits - one for Glanders and other for Equine Infectious Anaemia, developed by ICAR-National Research Centre on Equines, Hisar here today. Both the diseases are notifiable diseases in India and require special diagnosis to control and eradicate the Anaemia in the country. He also released a technology Bulletin, NRCE Profile and Research Outcome of NRCE.

Shri Radha Mohan Singh releases recombinant ELISA kits  Shri Radha Mohan Singh releases recombinant ELISA kitsShri Radha Mohan Singh releases recombinant ELISA kits

Smt. Krishna Raj, Union   Minister of State for Agriculture & Farmers Welfare; Dr. Trilochan Mohapatra , Secretary (DARE) & Director General (ICAR); Dr. J.K. Jena, Deputy Director General (Animal Science); Dr. Ashok Kumar, Assistant Director General (Animal Health); Dr. S. Honnappagol, Animal Husbandry Commissioner and Dr. B.N. Tripathi, Director, ICAR-NRCE also graced the occasion.

The Glanders is a fatal infectious and notifiable disease of equines that includes horses, donkeys and mules. The disease is caused by a bacterium known as Burkholderia Mallei and has zoonotic potential. The organism is also considered as potential bio-weapon and categorized under “Tier 1 Select Agent”. After continuous research efforts of more than 8 years, NRCE has been able to develop a recombinant Hep1 antigen ELISA as an alternate to Complement-Fixation Test (CFT). The ELISA has been duly validated in India and the OIE Reference Laboratory, Germany and showed excellent sensitivity (97.2%) and specificity (99.6%). This technology has been transferred to 8 State Disease Diagnostic Laboratories following approval of DADF, Ministry of Agriculture & Farmers’ Welfare and commercialized for transformation into ready to use kit. The ELISA has a huge potential of international commercialization as recombinant protein-based ELISA is not available in any other country. This technology will prove to be a milestone in controlling and eradicating Glanders from India.

The Equine Infectious Anaemia (EIA) is a chronic, debilitating and persistent infectious disease of equines caused by a retrovirus. It is an OIE notifiable disease. NRCE has also developed recombinant p26 protein-based ELISA as an alternative to Coggin’s test. This technology will provide sustainable and homogeneous source of antigen and harmonized protocol for ensuring regular surveillance of EIA. Both kits are highly economical as compared to imported kits.

(Source: ICAR-National Research Centre on Equines, Hisar)